Synthetic molecular oscillator could serve as a timer to control artificial cells
Researchers have made important discoveries regarding the behavior of a synthetic molecular oscillator, which could help create artificial cells.
A University of California, Riverside assistant professor of engineering is among a group of researchers that have made important discoveries regarding the behavior of a synthetic molecular oscillator, which could serve as a timekeeping device to control artificial cells.
Elisa Franco, an assistant professor of mechanical engineering at UC Riverside's Bourns College of Engineering, and the other researchers developed methods to screen thousands of copies of this oscillator using small droplets. They found, surprisingly, that the oscillators inside these small droplets behave in a very diverse way in terms of period, amplitude and phase.
"This diversity will play an important role in engineering complex behaviors in artificial cells, and we will take advantage of it," Franco said.
The paper, "Diversity in the dynamical behavior of a compartmentalized programmable biochemical oscillator," was published online this week in the journal Nature Chemistry. Co-authors were: Maximilian Weitz, Korbinian Kapsner and Friedrich C. Simmel, all of the Technische Universität München in Germany and Jongmin Kim and Erik Winfree, both of the California Institute of Technology.
Electronic oscillators are circuits that produce a periodic electronic signal, and they are used to regulate a variety of devices from radio and television transmitters to cellphones and computers. Biological systems are also regulated by complex molecular oscillators, from the level of individual cells up to entire organisms.
For decades, scientists have been trying to figure out ways to make artificial, programmable oscillators with molecules. Artificial oscillators may help adjust timekeeping in cells and regulating artificial cells. They could also be used as components in molecular computers that could create a middle ground between computers and nature.
Franco and the other researchers worked with a minimalistic artificial clock made with a soup-like mixture of DNA, RNA and proteins. An oil mixture was added to the oscillator mixture, and shaken to create an emulsion with thousands of water-in-oil droplets. Each droplet contained a copy of the oscillator mixture. Franco compared this simple emulsion preparation procedure to creating a salad dressing with oil and vinegar.
While emulsions are a common laboratory technique used to generate many small samples and run thousands of experiments in parallel, not many studies have looked at dynamic circuits like oscillators.
In the experiments outlined in the Nature Chemistry paper, the "blinking" droplets containing the oscillator were studied under a microscope, where the diverse behavior of amplitude and frequency was observed.
Through mathematical modeling, the researchers were able to attribute this diversity to uneven distribution of molecules inside each droplet, a phenomenon called "partitioning noise." This variability is a major challenge as well as an opportunity for the development of artificial cells. Similar experiments will allow scientists to optimize artificial molecular devices so they are minimally affected by partitioning noise.
Funding for Franco's research comes from the National Science Foundation, Bourns College of Engineering at UC Riverside and the UC Regents Faculty Development Fellowship.
Bourns College of Engineering
- Edited by CFE Media. See more Control Engineering sensor and vision stories.
|Search the online Automation Integrator Guide|
Case Study Database
Get more exposure for your case study by uploading it to the Control Engineering case study database, where end-users can identify relevant solutions and explore what the experts are doing to effectively implement a variety of technology and productivity related projects.
These case studies provide examples of how knowledgeable solution providers have used technology, processes and people to create effective and successful implementations in real-world situations. Case studies can be completed by filling out a simple online form where you can outline the project title, abstract, and full story in 1500 words or less; upload photos, videos and a logo.
Click here to visit the Case Study Database and upload your case study.